Abstract
Summary Germinal-vesicle-breakdown (GVBD) of Xenopus laevis oocytes was induced by injecting 132,000 g supernatant of Tetrahymena pyriformis homogenates into oocytes. Using induction of GVBD as a bioassay system, we succeeded in purifying two GVBD-inducing proteins from the 132,000 g supernatant by liquid chromatography with an anion-exchange MonoQ column, ultra-filtration, and electroelution from bands on a native-PAGE gel. A protein of 40 kDa induced GVBD in the absence of protein synthesis of Xenopus oocytes whereas another protein of 56 kDa induced GVBD only in the presence of the oocyte protein synthesis. These proteins did not react with the PSTAIR antibody, which is specific for cdc2 homologues.
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