Abstract
Fresh horse-pituitary glands were extracted with 40% ethanol and the gonadotropins were recovered by increasing the alcohol concentration to 85% followed by drying with acetone. This preparation was further extracted with water at pH 5, and the extract was adjusted to pH 7 and lyophilized. The follicle-stimulating hormone in the pH-5-souluble fraction was purified by zone electrophoresis and resolved into six components by starch-gel electrophoresis. One of these components contained follicle-stimulating hormone which was recovered in the elution cell and the contaminating starch was separated by chro,atography on Dowex- 2 X8. This follicle-stimulating hormone stimulated estrogen production in immature hypophysectomized female rats and was essentially free of luteinizing hormone and other pituitary hormones. It migrated as a single zone in starch-gel electrophoresis and sedimented as a single boundary in the ultracentrifuge.
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