Abstract
Publisher Summary Rabbit liver is an unusually rich source of enzymes that utilize tetrahydrofolate (THF) as a coenzyme. Several of these enzymes have proved useful in analyzing THF pools, as well as in understanding the role and mechanism of THF in one-carbon metabolism. In addition to being a rich source of enzymes, rabbit liver can be obtained in relatively large quantities at modest cost from Pel-Freeze Biologicals. This chapter discusses purification of folate-dependent enzymes from rabbit liver. This enzyme activity decreases about 10–15% per week and should be purified within a few days after receiving the livers. The physiological form of THF contains a chain of glutamate residues linked through the γ-carboxyl group. To accommodate the naming of these derivatives, they are named as polyglutamate derivatives of tetrahydropteroate (H 4 Pte), which has no glutamate residue. Tetrahydrofolate thus becomes H 4 PteGlu and the pentaglutamate form becomes HnPteGlus.
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