Abstract
In order to obtain high-purity flavonoid products, the extracts from mulberry leaves were separated and purified via high-speed counter-current chromatography (HSCCC). Moreover, the product was detected via high-performance liquid chromatography (HPLC). The characteristic absorption wavelength of the rutin standard for HSCCC detection and HPLC analysis at 257 nm was tested by ultraviolet scanning analysis. The effect of solvent systems and mobile phase flow rate on the separation efficiency were then researched. Finally, the solvent system of V(ethyl acetate):V(n-butanol):V(water) = 4:1:5 was selected as the operating system for HSCCC. This work theoretically analyzed the impact of the molecular structure and polarity of flavonoids on the choice of solvent systems. The results showed that the mobile phase flow rate had a great influence on the separation efficiency. Furthermore, the separation efficiency increased as the mobile phase flow rate decreased. When the mobile phase flow rate was 5 mL/min, the peak time for flavonoids was 140 min, the retention of the stationary phase was 56.4%, and the purity of the product reached 93.8%. The results of this study greatly improved the purity of flavonoids in mulberry leaf and provided a strong support for the separation and purification of mulberry leaf extract.
Highlights
IntroductionMulberry leaves are rich in active compounds, as they contain a variety of vitamins, amino acids, polysaccharides, flavonoids, alkaloids, and a large number of essential microelements [1,2]
Mulberry leaves are rich in active compounds, as they contain a variety of vitamins, amino acids, polysaccharides, flavonoids, alkaloids, and a large number of essential microelements [1,2].These plants are cultivated in most areas of China and have a very high medicinal value, demonstrating positive effects on clearing the liver and eyes, lowering blood pressure, correcting blood lipid and blood sugar levels, as well as countering hypertension and virus infections [3,4,5]
When separating and purifying flavonoids from mulberry leaf extracts via high-performance liquid chromatography (HPLC), rutin is typically used as the standard
Summary
Mulberry leaves are rich in active compounds, as they contain a variety of vitamins, amino acids, polysaccharides, flavonoids, alkaloids, and a large number of essential microelements [1,2]. These plants are cultivated in most areas of China and have a very high medicinal value, demonstrating positive effects on clearing the liver and eyes, lowering blood pressure, correcting blood lipid and blood sugar levels, as well as countering hypertension and virus infections [3,4,5]. Ion exchange resins have the advantages of a high exchange capacity, low regenerant consumption, and long service life
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