Abstract
Objective To purify early pregnancy factor (EPF)from the sera of pregnant women with immunosuppressive conditions. Methods Forty women in Taiyuan, Shanxi, China were chosen to detect ovulation by ultrasonagraphy daily.20 to 40 mL of blood was collected after 1 to 8 days of fertilization.400 mL sera from these women were subjected to the detection of EPF activity by rosette inhibition test(RIT ). EPT was purified by DEAE Sepharose ion-exchange codumn, Con A sepharose 4b and Heparin Sepharose CL-6B. Result EPF was detected in the blood of pregnant women at 1 to 8 day after fertilization.EPF was purified by chromatography with molecular weight of 18 000. Conclusion Purification of EPF in the stage of periimplantation will provide the valuable significance of theoretical and clinical usage for the research of maternal immunologic tolerance of the allogenic fetus. Key words: Periimplantation; Early pregnancy factor; Separation; Purification
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