Abstract
DNAs from the endosymbiotes of two species of grain weevils, Sitophilus oryzae and S. zeamais, were separated from host-weevil DNA and purified by cesium chloride gradient centrifugation. Endosymbiote DNA was identified by hybridization with a probe of the gene encoding the 16S rRNA of Escherichia coli. Estimations of the CsCl buoyant densities of symbiote DNAs of S. oryzae and S. zeamais were similar ( ϱ = 1.712 and 1.714, respectively) corresponding to a mole per cent guanine + cytosine (GC%) of ≈52–55%. The GC%s of host-weevil DNAs were ≈33%.
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