Abstract
Summary A stable complement-fixing antigen of Rickettsia orientalis, free of anti-complementary substance, was obtained by sodium deoxycholate (DCA) sucrose extraction of yolk sac suspensions previously treated with Amberlite XE-64. The optimum concentration of DCA, 1.0% in 0.25 M sucrose solution, yielded an antigen containing approximately one tenth of the nitrogen of the material extracted by sucrose alone without any change in the complement fixation titer of antigen. The DCA-extracted antigen was stable on storage at -70°C for periods of 5 months or more.
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