Abstract
High-speed counter-current chromatography (HSCCC) is applied to the purification of coenzyme Q 10 (CoQ 10) for the first time. CoQ 10 was obtained from a fermentation broth extract. A non-aqueous two-phase solvent system composed of heptane–acetonitrile–dichloromethane (12:7:3.5, v/v/v) was selected by analytical HSCCC and used for purification of CoQ 10 from 500 mg of the crude extract. The separation yielded 130 mg of CoQ 10 at an HPLC purity of over 99%. The overall results of the present studies show the advantages of HSCCC over an alternative of silica gel chromatography followed by recrystallization. These advantages extend to higher purity (97.8% versus 93.3%), recovery (88% versus 74.3%) and yield (26.4% versus 23.4%). An effort to avoid the toxic, expensive solvent CH 2Cl 2 was unsuccessful, but at least its percentage is low in the solvent system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
