Purification of cell membrane glycoproteins by lectin affinity chromatography

Abstract

1. Introduction 330 I1. (Tell surface membrane organization and dynamics 330 1II. Cell membrane glycoproteins 332 A. Saccharide components ofglycoproteins 332 B. Glyeophorin as a model for integral membrane glycoproteins 334 IV. Lectins and their saccharide-binding properties 336 V. Approaches useful for the purification and characterization of cell membrane lectin- binding components 338 A. General considerations 338 B. Cell surface labeling of membrane proteins and glycoproteins 339 VI. Non-affinity methods of cell membrane glycoprotein purification 340 A. Isolation and characterization of lectin-binding glycoproteins 340 B. Isolation and characterization of lectin-reactive glycoproteins 342 1. Solubilization of membrane integral proteins 342 2. Purification of solubilized membrane glycoproteins by conventional procedures . 343 VII. hnmobilized lectin derivatives useful for affinity chromatography 344 VIII. Applications of immobilized lectins for fractionation and purification of membrane gly- coproteins 347 A. Erythrocyte membrane glycoproteins 347 B. Lymphocyte plasma membrane glycoproteins 35 l C. Neural glycopeptides and glycoproteins 357 D. Membrane glycoproteins from untransformed and transformed cells 360 E. Membrane glycoproteins isolated from miscellaneous biological materials ....... 368 IX.