Abstract
Abstract The C′3 inactivator was reported to be a naturally-occurring serum factor which inactivates C′3 in EAC′1423 as measured by immune hemolysis and immune adherence. The inactivator was purified from human serum by column chromatography on diethylaminoethyl (DEAE)-cellulose and carboxymethyl (CM)-cellulose followed by Pevikon C-870 zone electrophoresis and Sephadex G-200 superfine gel filtration. A sample of C′3 inactivator was analyzed by ultracentrifugation. A single peak was observed, which remained symmetrical throughout sedimentation, with an S20,w = 5.2 S. In sucrose density gradient ultracentrifugation the C′3 inactivator was distributed in a narrow symmetrical peak with an S value of 5.2. Purified preparations of C′3 inactivator gave a single precipitin line in the β1 globulin region in immunoelectrophoresis. C′3 inactivator has been found to have several biologic functions. This substance inhibits 1) the passive cutaneous anaphylaxis (PCA) reaction, 2) the generation of anaphylatoxin, 3) the rejection of skin homografts, 4) immunophagocytosis and 5) the Prausnitz-Küstner reaction.
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