Abstract
A simple and rapid means for purification of three animal viruses is described. Vesicular stomatitis virus, vesicular exanthema virus, and infectious rhinotracheitis virus were all precipitated quantitatively with Zn(OH) 2. The zinc hydroxide-virus complex was then dissociated by ethylene-diaminetetraacetic acid. Approximately 90% of the nonspecific nitrogen was eliminated by this method from crude vesicular stomatitis virus suspensions. There appears to be a direct relationship between concentration of Zn(OH) 2 and the amount of vesicular stomatitis virus recovered. The virus was removed quantitatively by 0.05% Zn(OH) 2 from a preparation containing 10 7 plaque-forming units per milliliter. A high-titered preparation of vesicular exanthema virus was obtained after precipitation by Zn(OH) 2 and subsequent high-speed centrifugation. An electron micrograph of the purified preparation revealed spherical particles 55 mμ in diameter. The ratio of these particles to plaque count was approximately 200:1. The observed sedimentation rate of about 140 Svedberg units is compatible with the size shown by electron microscopy.
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