Abstract
An extracellular proteolytic enzyme from the culture medium of Penicillium notatum, obtained as a dry, tannin‐precipitated powder from a commercial source, has been isolated. The purification procedures used include extraction of the crude proteinase with buffer from the commercial product, precipitation with ammonium sulphate, chromatography on DEAE‐Sephadex A‐25, gel filtration on Sephadex G‐75, followed by ion‐exchange chromatography on SE‐Sephadex C‐50, and finally zone electrophoresis on a cellulose column. Sufficient evidence is presented to show the purity of the isolated proteinase. Arguments for and against the presence of two distinct proteolytic enzymes from the same source are also presented.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.