Abstract
Pyrenophora tritici-repentis, causal agent of tan spot of wheat, produces a toxin in culture that induces necrosis only on wheat cultivars susceptible to the pathogen. The toxic compound, named Ptr toxin, was purified by gel filtration chromatography and ion exchange chromatography. Unlike most other fungal phytotoxins, Ptr toxin is a low molecular weight protein of 14,700. Reduction with dithiothreitol abolished toxic activity indicating that cysteine is present and that a disulfide bond is required for activity
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