Abstract
Abstract An acidic calcium-binding phosphoprotein has been isolated from beef adrenal medulla and purified to apparent homogeneity. The purification procedure employed ammonium sulfate fractionation, DEAE-cellulose chromatography, and gel filtration on Sephadex G-100 and G-50. This protein, which appears to be the only soluble calcium-binding protein in adrenal medulla, shows high affinity calcium binding (Kd = 1.7 x 10-5 m) and binds 1 mole of calcium per mole of protein. Each mole of calcium-binding protein contains 1 mole of phosphate. The molecular weight was determined to be 11,900 by equilibrium ultracentrifugation. An acidic calcium-binding phosphoprotein was also isolated from beef brain and compared with the protein from beef adrenal medulla. The two proteins appear to be identical on the basis of electrophoretic migration in 15% acrylamide gels and 10% acrylamide gels containing sodium dodecyl sulfate, molecular weight, amino acid composition, and peptide maps. The protein is not found in other tissues including liver, kidney, lung, and parotid gland. It is suggested that this calcium-binding protein of brain may be associated with adrenergic neurons.
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