Abstract
AbstractThe fatty acid moiety of 2‐monoacyl‐sn‐glycerol (2‐MAG) undergoes spontaneous acyl migration to the sn‐1(3) position, resulting in a thermodynamic equilibrium of approximately 1:9 of 2‐MAG to 1(3)‐monoacyl‐sn‐glycerol (1‐MAG). Spontaneous acyl migration is an impediment to synthesizing and isolating specific 2‐MAG for use as intermediates in the synthesis of structured triacylglycerols. 2‐Monooleoyl‐sn‐glycerol was synthesized by the enzymatic ethanolysis of triolein and isolated by liquid CO2 extraction. The resultant MAG, diacylglycerol, and fatty acid ethyl esters were quantified by 1H NMR and supercritical fluid chromatography. The low polarity of the CO2 and mild extraction temperature (25 °C) resulted in very low spontaneous acyl migration rates, allowing the MAG to be isolated in 80% yield and in a very high 2‐MAG:1‐MAG ratios of ≥93 mol%.
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