Abstract
The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). GC–MS and methylation analysis that linkages were mainly β-1,3 and β-1,6, β-1,4 and β-1,2 bonds in WIP. Seven main oligomer products were detected and their structures characterized by mass spectrum. Experimental results shown the similarity in structure between water soluble polysaccharides (WSP) and water insoluble polysaccharides (WIP), thus WSP can be the product of cell wall by breakdown.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Biological Macromolecules
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
