Abstract
In this study, an endo-1,4-β-xylanase was purified from wheat malt following the procedures of ammonium sulfate precipitation, cation-exchange chromatography, and two-step anion-exchange chromatography. The purified endo-1,4-β-xylanase had a specific activity of 3.94 u/mg, demonstrating a weight average molecular weight (Mw) of approximately 58,000 Da. After LC–MS/MS (Liquid chromatography-tandem mass spectrometry) identification, the purified enzyme had the highest matching degree with a GH10 (Glycoside Hydrolase 10) domain-containing protein from wheat, there were 23 match peptides with a score above the threshold and the prot-cover was 45.5%. The resulting purified enzyme was used to investigate its degradation ability on high viscosity wheat-derived water-extractable arabinoxylan (WEAX). Degradation experiments confirmed that the purified enzyme was a true endo-acting enzyme, which could degrade large WEAX into smaller WEAX. The average degree of polymerization (avDP) and the viscosity of WEAX decreased with the increasing reaction time. The enzyme could degrade a small amount of WEAX into arabinoxylan-oligosaccharides (AXOS) with a degree of polymerization of 2–6, but no monosaccharide was produced. The degradation occurred rapidly in the first 3.5 h and decreased with the further prolongation of reaction time.
Highlights
Nowadays, wheat beer is gaining popularity in China, and even in other countries of the world, because of its mellow taste and rich and lasting foam
At least 40% of wheat malts are used in the production of wheat beer
Arabinoxylan can be classified into water-soluble arabinoxylan (WEAX) and water-insoluble arabinoxylan (WUAX) based on their solubility [5]
Summary
Wheat beer is gaining popularity in China, and even in other countries of the world, because of its mellow taste and rich and lasting foam. At least 40% of wheat malts are used in the production of wheat beer. The demand for wheat malt is increasing due to the prevalence of wheat beer in recent years [1]. The presence of a high amount of macromolecule polysaccharide in wheat can decrease the beer filtration rate [2], increasing the production cost of beer. Arabinoxylan (AX) is one of the main components in the endosperm cell walls of wheat [3], which has a linear backbone of (1,4)-β-D-xylopyranose with α-L-arabinofuranose substitutions [4]. The content of AX in wheat is about 3–12.5% [6]. The content of WEAX in wheat malt is
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