Abstract
PCNA is a ring-shaped protein that encircles DNA and is essential for DNA metabolism, including DNA replication and repair. PCNA is either a homotrimer in eukaryotes and euryarchaeotes or a heterotrimer in some crenarchaeotes. The crenarchaeon Sulfolobus solfataricus encodes three PCNA homologues (PCNA1, PCNA2, and PCNA3). PCNA1 and PCNA2 form a stable dimer. The dimer then recruits PCNA3 to form the trimeric ring-shaped molecule that is typical for all PCNA proteins. We crystallized the PCNA3 monomer, the PCNA1–PCNA2 heterodimer, and the PCNA1–PCNA2–PCNA3 heterotrimer. The crystals diffract X-ray to 1.9, 2.6, and 2.5 Å resolutions, respectively. SAD phasing and molecular replacement solutions have confirmed that the crystals do contain the corresponding monomer, dimer, and trimer.
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