Abstract

Tannase produced extracellularly by the bacterial strain Bacillus haynesii SSRY4 MN031245 was purified in step-wise manner through ammonium sulphate precipitation, dialysis, followed by anion exchange chromatography. Tannase was purified to 42.0-fold with 36.30% enzyme yield. The enzyme was relatively stable from 30 to 50℃ and pH (4.0–6.0) for up to 4 hours. Partially purified tannase (16.80 U/ml) was able to synthesize 20.304 mg/ml gallic acid from the fruit waste under optimized conditions. The results of application study suggest that bacterial tannase could provide a new source for Gallic acid synthesis from the fruit waste for industrial applications. Our research findings could provide a value chain to fruit waste and help in reducing the waste generation from fruit processing industries.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.