Abstract
Crude mitochondria isolated from Jerusalem artichoke (Helianthus tuberosus L.) tubers were purified on a 23% (v/v) continuous Percoll gradient. Microbodies and damaged mitochondria banded on top of the gradient, whereas the purified mitochondria handed close to the bottom. The purified mitochondria showed improved membrane integrities, specific enzyme activities and respiratory properties (higher rate, respiratory control, ADP/O ratio) than the crude mitochondria. Purified mitochondria could he stored for 24 h on ice in a phosphate buffer with only small loss of activity.
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