Abstract
BackgroundAmaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity.MethodsIn this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro.ResultsWe found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe3+ was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc).ConclusionThe in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.
Highlights
Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family (Akubugwo et al, 2007; Das, 2016)
Cheng et al (2015) found that the fruit of Schisandra chinensis Baill polysaccharide was extracted by different methods, microwave-assisted extraction (MAE) was more efficient than enzyme-assisted extraction (EAE), Ultrasonic-assisted extraction (UAE) and Heat reflux extraction (HRE)
The results showed that AHP-M-2 exhibited stronger antioxidant activity than AHP-M-1 against DPPH, superoxide radical and hydroxyl radicals, which could be related to differences in the protein content, monosaccharide composition and chemical structure
Summary
Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family (Akubugwo et al, 2007; Das, 2016). This plant is often used as a vegetable to treat intestinal bleeding, diarrhea and excessive menstruation. Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L. Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. Conclusion: The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants
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