Abstract
A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.
Highlights
Oxidative stress-induced cell damage triggers both the physiological process of aging and many pathological progressions that can eventually lead to serious health problems [1]
The results indicated that mannose and glucose were the major monosaccharides forming the backbone of BEBP. The polysaccharide (BEBP)-1
It was concluded that the water-extracted crude polysaccharide BEBP from Boletus edulis Bull could be purified by DEAE-cellulose column chromatography
Summary
Oxidative stress-induced cell damage triggers both the physiological process of aging and many pathological progressions that can eventually lead to serious health problems [1]. Research on antioxidants, especially exploration of potent natural compounds with low cytotoxicity from plants, has become an important branch of biomedicine. Previous studies have indicated that the polysaccharides in plants are energy resources, but play key biological roles in many life processes as well. The structure and mechanisms of pharmaceutical effects of bioactive polysaccharides on diseases have been extensively studied, and more natural polysaccharides with different curative effects have been tested and even applied in therapies [3]. Recent researches exhibited that some polysaccharides have been demonstrated to play an important role as free radical scavenger for the prevention of oxidative damage in living organisms [4,5]. There are abundant polysaccharides in Boletus edulis Bull [6]. Boletus edulis [7], but the purification and antioxidant ability in vitro and in vivo of the polysaccharide from Boletus edulis Bull has not been reported, the purpose of the present investigation was to elucidate the isolation and characterization of water-soluble polysaccharide from Boletus edulis Bull, as well as to evaluate its antioxidant activities in vitro and in vivo
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
