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Abstract
1. 1. A β-glucosidase (β- d-glucosido glucohydrolase, EC 3.2.1.21) was purified from xylophageous larvae of the Cerambycidae, Phoracantha semipunctata, by ammonium sulfate precipitation, hydroxylapatite and DEAE-cellulose chromatography. 2. 2. On an acrylamide gel, the enzyme appeared as a single peak of protein. Molecular weight was approx. 100,000. 3. 3. The pH optimum was between 5 and 5.5 and the K m value 2.2 mM for p- nitrophenyl-β- d-glucopyranoside . 4. 4. The thermal stability of the enzyme and the effect of various cations and monosaccharides on activity were examined. 5. 5. The enzyme hydrolyzes various alkyl and aryl-β- d-glucosides, also cellobiose, but neither carboxymethyl cellulose nor cellulose. 6. 6. The main physiological rôle of the enzyme presumably consists in hydrolyzing the cellobiose liberated from the ingested wood by the degradation of the cellulose by the cellulasic complex in the midgut of the Phoracantha semipunctata larvae.
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