Abstract
Bambusa pervariabilis × Dendrocalamopsis grandis blight is caused by a toxin from the fungus Arthrinium phaeospermum (corda) M. B. Ellis. We used shaking culture in a modified Fries culture medium and methanol extraction to isolate the toxin. The optimal developing solvent mixture (methanol: ethyl acetate: H2O at 7:1.5:3) was selected using thin layer chromatography and used as the eluent for toxin purification by silica gel column chromatography. Two toxic fractions were identified in the bioassay. A flaxen oil substance, AP-I, showed higher toxicity than a toxic white powder, AP-II. The more toxic AP-I was determined to be dibutyl phthalate (C16H22O4, molecular weight of 278) by mass spectrometry, nuclear magnetic resonance, and infrared spectrophotometry. Dibutyl phthalate might contribute to the pathogenesis of bamboo blight.
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