Abstract
Microbial secondary metabolites produced by actinomycetes are important natural products widely applied to control plant diseases. A variety of actinomycetes were isolated from soil samples collected from Tianzhu Mountain in Shenyang, China. A Streptomyces strain Shenyang Tianzhu (STZ) exhibits effective antiviral activity against Tobacco mosaic virus (TMV). The isolate was identified as Streptomyces ahygroscopicus based on its cultural, morphological, physiological, biochemical characteristics as well as the phylogenetic analysis using 16S rRNA sequences. To obtain the pure anti-TMV compound from Streptomyces STZ, the culture broth was subjected to Amberlite IRC-50 ion-exchange resin, SX-8 macroporous adsorption resin and Sephadex G-25 gel column chromatography. The purified active compound was confirmed to be ε-poly-l-lysine (ε-PL), with molecular mass in the range of 3454–4352 Da by structural analysis with infrared (IR), matrix-assisted laser desorption ionization-time-of-flight MS (MALDI-TOF), thin-layer chromatography (TLC) and high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR). The protective and curative effects of the purified compound ε-PL were tested and the results showed that the compound exhibited significant protective and curative activity against TMV. The potential application of ε-PL as an efficient anti-plant virus agent was expected.
Highlights
Plant virus diseases cause considerable economic losses in cereal and horticulture production worldwide every year [1]
China, which was produced by Streptomyces ahygroscopicus isolated from soil samples of Liaoning by Streptomyces isolated from soil samples of Liaoning
Tobacco mosaic virus (TMV) using and the stability subculture.strains using the isolated isolated in Streptomyces wereactivities assessedofbyfermentation the half-leafcultures methodagainst and the results indicated that
Summary
Plant virus diseases cause considerable economic losses in cereal and horticulture production worldwide every year [1]. Plant virus inhibitors from metabolites of microbes have been considered as a potential alternative for chemical pesticides, which have become an increasing research spotlight These bio-agents have various advantages in the respect of low mammalian toxicity, biodegradability, good environmental compatibility and a unique mode of action [4]. Ningnanmycin is an effective anti-plant virus agent that was isolated from the fermentation broth of Streptomyces noursei var. China, which was produced by Streptomyces ahygroscopicus isolated from soil samples of Liaoning by Streptomyces isolated from soil samples of Liaoning It displays a broad antiviral activity against many plant viruses, such It asdisplays. China,Streptomyces and showed strain significant anti-TMV activity. Application of ε-PL as an efficient anti-plant virus agent was proposed first time
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