Abstract
Tannase (tannin acyl hydrolase, EC 3.1.1.20) was purified from the culture broth of Candida sp. by rivanol fractionation and chromatography of columns of ECTEOLA-cellulose, Sepharose 6B and Sephadex G–200. The purified tannase was homogeneous on SDS-polyacrylamide gel electrophoresis. The stable pH range of the enzyme was 3.5 to 7.5. The optimum pH was 6.0 for gallotannin as a substrate. The enzyme was stable up to 40°C. The yeast tannase hydrolyzed the ester and depside linkages of tannic acid. The enzyme activity was not influenced by various metal ions and EDTA.
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