Abstract
Several attempts to purify m-RNAs have already been made mostly using bacterial cells (1, 2). Experiments using Hela cells (3), red blood cells (4–7), mammalian liver tissue (8–11) and thymus (12) have lead to the isolation of RNA preparations, the messenger character of which was detected by DNA-like base composition, competence to stimulate amino acid incorporation into proteins or hybridization with homologous DNA (13, 14).This note summarizes results obtained in the purification of sheep thyroid and rat liver RNAs. The stimulation of incorporation of L-leucine-14C into protein material by a cell-free extract from E.coli was used as a test for m-RNA activity. E.coli RNA was used a reference material.
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