Abstract
A factor inhibiting the leukocytosis-promoting activity of parotin was isolated from rabbit serum and the nature of this factor was examined, with following results. 1) Inhibition of the activity by rabbit serum was observed by treatment at 37°for 15 min, and a definite inhibition was seen when treated for over 60 min. 2) This parotin-inhibitory factor is stable to heating at 56°for 30 min but loses its activity when heated at 100°for 5 min. 3) This inhibitory factor was purified by ammonium sulfate fractionation, gel filtration over Sephadex G-200 and Sepharose 6B, chromatography over DEAE-cellulose, and isoelectric focusing in carrier ampholyte, and a substance homogeneous in disc electrophoresis and isoelectric focusing in carrier ampholyte was separated. The molecular weight of this substance was 210000 by gel filtration over Sephadex G-200, 190000 by gelfiltration over Sepharose 6B, and 160000 by the Hedrick-Smith method. It had an isoelectric point at pH 5.8. 4) This parotin-inhibitory factor seemed to have an inhibitory action different from antigen-antibody reaction or proteinolytic reaction.
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More From: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
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