Abstract
Cytochrome c550 was purified to electrophoretic homogeneity by ion-exchange chromatography and gel filtration from a soluble fraction of Flavobacterium saccharophilum. The purified cytochrome c550 had a molecular weight of 12500 as determined by gel filtration and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Cytochrome c550 possessed absorption peaks at 407 nm in the oxidized form, and at 415, 521, 550nm in the reduced form, Soluble D-glucoside 3-dehydrogenase reduced the purified cytochrome c550 with sugar, and this sugar was converted to the corresponding 3-keto sugar. The purified cytochrome c550 was autoxidizable. This shows that cytochrome c550 may be an in vivo electron acceptor for soluble D-glucoside 3-dehydrogenase.
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