Abstract
Chilo iridescent virus (CIV) purified from larvae of Galleria mellonella and Chilo suppressalis by differential and density gradient centrifugations exhibited at least three chromatographic components separated on the diethylaminoethyl cellulose columns. Two additional components which were eluted with lower concentration of salt solution were obtained when the infected larvae had been stored at 4°C over 2 weeks. Unfractionated CIV preparations treated with pancreatic phospholipase gave a homogeneous population which was readily eluted from the cellulose column at low salt concentration. Similar chromatographic heterogeneity was observed with the centrifugally homogeneous preparation of Tipula iridescent virus (TIV). The separated CIV components showed no differences in infectivity and gross physical and chemical properties. The difference in particle size between CIV (160 mμ) and TIV (130 mμ) is consistent with the difference in sedimentation rate between the two viruses.
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