Abstract
As part of a study to determine the variation in the effectiveness of commercial pesticide formulations of ester steroidal alkaloids from sabadilla (Schoenocaulon officinale Grey) seeds in the integrated pest management of citrus, a method to isolate and measure large quantities of alkaloids from natural starting materials was needed. The addition of the solvent modifier sodium dodecyl sulfate (SDS) to a previously published HPLC method greatly improved peak resolution by minimizing peak tailing. The use of the non-ester alkaloid papaverine as an internal standard permitted quantification of sabadilla alkaloids by HPLC, and this modified method was then used to monitor the breakdown of cevadine and veratridine, the two most abundant sabadilla alkaloids, in aqueous solutions as a function of time and pH.
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