Abstract
The vitellogenin (VG) and vitellin (VN) of Locusta migratoria have been purified from ovariectomized female haemolymph and from oocytes, respectively, by a new procedure. The products are free of lipoprotein I, which was present as a contaminant in previous preparations. The isoelectric point of VG and VN is pH 6.3. The amino acid compositions of VG and VN do not differ significantly. The lipid moiety (8.2% by weight of VG and 6.7% by weight of VN) includes diacylglycerol, a trace of triacylglycerol, cholesterol, phosphatidylcholine and phosphatidylethanolamine. The content of diacylglycerol is much lower in VN than in VG, which suggests the release of lipid within the oocyte.
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