Abstract
Tomato spotted wilt virus was purified by chromatography on columns of calcium phosphate, precipitation with polyethylene glycol, density gradient centrifugation, and ascending zone electrophoresis in a sucrose density gradient. Examination of the virus by electron microscopy revealed particles of different shapes in different suspending media. Particles fixed with glutaraldehyde were spherical and somewhat flattened and had a corrected diameter of 85 nm. Antisera with a titer of 1 128 against the virus were prepared which did not react with plant antigens. The virus had a sedimentation coefficient s 20, w of 530 S and, when fixed in 0.5% glutaraldehyde, it had an electrophoretic mobility at pH 7 of −19.8 × 10 -5 cm 2 V −1 sec −1.
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