Abstract
A purification procedure involving chromatofocusing by means of which toxic components may be obtained in a pure form from crude egg extracts of the tick species Rhipicephalus evertsi evertsi, Hyalomma truncatum, Boophilus microplus and B. decoloratus is described. The molecular weight of the toxin from R. evertsi evertsi was shown to be approx. 5500 according to sedimentation equilibrium centrifugation, SDS gel electrophoresis and calculations from the amino acid composition. The mol. wt of the toxins from the other tick species were found to be between approx. 27,000 and 40,000. Large differences in the amino acid composition of the toxins exist. The pIs range from 6 in the case of R. evertsi evertsi to 9.2 for B. decoloratus. The MLD of the toxins are between 0.4 and 1.7 mg/kg as determined by subcutaneous injection into guinea-pigs. The histopathological symptoms caused by the crude egg extracts of purified toxins of the different tick species appear to be the same in guinea-pigs. Lesions include focal areas of necrosis in the liver, mineralization and oedema of the urinary bladder and vacuolation of the lining epithelium.
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