Abstract
The isolation and characterization of glycogen-type polysaccharide from the Sclerotia of Corticium rolfsii are described. The purified polysaccharide ([α]D+198°) was shown to be homogeneous by zone electrophoresis and ultracentrif ugation. The molecular weight, estimated by gel filtration, was larger than 2.0×106 and the sedimentation coefficient was 5 .39×10-12 The results from enzymatic hydrolysis with various amylases (α-, β- and isoamylase) and methylation analysis indicate that the polysaccharide was a highly branched glycogen. The average chain length of this glycogen was calculated to be 8, and the exterior and interior chain length were 5 and 2, respectively.
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