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Abstract
Crystalline human ceruloplasmin was prepared from a Cohn's fraction F‐IV‐1 of normal human pooled plasma, by procedures based on acetone fractionation and chromatography on DEAE‐sephadex A‐50.Needle‐shaped crystals of the human ceruloplasmin showed a single component on ultracentrifugation, electrophoresis and immunoelectrophoresis and had a sedimentation constant of 7.04 S and an electrophoretic mobility of 5.2 × 10–5 cm2 volt‐1 sec‐1. Light scattering of the ceruloplasmin gave a molecular weight of 160,000. Absorption coefficient at 610 nm was E1%1 cm 0.66, at 280 nm, 14.7 and the ratio E610 nm/E280 nm, 0.045. This protein provided 0.31% copper and contained serine, aspartic acid and valine residues as an amino terminal amino acid and showed an oxidase activity of 62 arbitrary units.
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