Abstract
1. 1. Neuraminidase ( N -acetylneuraminate glycohydrolase, EC 3.2.1.18) from the chorioallantois of the 19-day-old chick embryo has been isolated and studied. 2. 2. The isolation procedure consisted of mild extraction of the washed membranes, heat treatment, precipitation with ammonium sulphate and chromatography on a column of hydroxyl apatite. The final product is about 200-fold purer than the starting fluid. 3. 3. The purified preparation is stable at o° but unstable at 37°. It has a sedimentation coefficient of about 3.3 S and is inactivated by sulfhydryl reactants. The pH of optimum activity on three substrates, sialyl lactose, fetuin and ovine submaxillary gland mucin is between 5 and 4. Values for K m and maximum rates of action on these substrates have been determined.
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