Abstract
An acid ribonuclease (RNase RCL2) was purified to homogeneity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) from a homogenate of bullfrog liver (Rana catesbeiana). The apparent molecular weight estimated from SDS-PAGE was ca. 25kDa. The pH optimum of the RNase was 5.0. The RNase released mononucleotides from RNA in the order of 3'-UMP, 3'-GMP and 3'-AMP. The N-terminal amino acid sequence of RNase RCL2 was determined up to the 20th residue, and it was found to have a 5 residue sequence homology with that of oyster acid RNase [H. Watanabe et al. J. Biochem. (Tokyo), 114, 800 (1993)]. Thus, RNase RCL2 seems to be a member of the RNase T2 family RNases. This is the first evidence of the RNase T2 family RNase in amphibians.
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