Purification and preliminary characterization of phosphoglycerate mutase from Schizosaccharomyces pombe

Abstract

1. 1. Phosphoglycerate mutase could be purified to over 95% homogeneity by a single step procedure involving elution from Cibacron Blue-Sepharose by a pulse of cofactor 2,3-bisphosphoglycerate. 2. 2. Although the enzyme has been isolated in only small quantities ( c. 100 μg), gel filtration and sodium dodecylsulphate polycrylamide gel electrophoresis indicated that it is monomeric with M r approximately 23,000, an extremely low value for this enzyme. 3. 3. Preliminary investigations of the kinetic characteristics and the nature of important amino acid side chains have been undertaken.