Abstract
The red alga Gracilaria verrucosa was found to possess hemagglutinins against various kinds of animal erythrocytes; among these the horse, guinea pig, and chicken erythrocytes were strongly agglutinated. The hemagglutinins were very stable between pH 4 and 12 and also relatively stable to heat. Their activity was not affected by the divalent metal cations tested. One hemagglutinin component (GVA-1) was purified by successive column chromatographies on DEAE-cellulose, Sephadex G-75, Hydroxyapatite, and Sephadex G-75 again. GVA-1 was a tetramer which was comprised of two kinds of subunits (MW 12, 000 and 10, 500); its molecular weight was about 41, 000. It contained high amounts of glycine and hydroxyl amino acids (threonine and serine) but no half cystine. The content of acidic amino acids (aspartic acid and glutamic acid) was fairly larger than that of basic amino acids (lysine, histidine and arginine); it coincided with the low isoelectric point, 4.8, of GVA-1. None of the sugars used inhibited the activity of GVA-1.
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