Abstract
Sindbis virus multiplies rapidly in chick fibroblast monolayers and yields high titers in the extracellular fluid before any cytopathic effect is evident and before cells become detached from the glass. Purification of the virus can therefore be undertaken from tissue culture fluid relatively uncontaminated by cellular debris. A simple purification procedure based on adsorption to AIPO 4 gel and differential centrifugation is described. The purified virus was found to be homogeneous by chromatography and by density gradient centrifugation in sucrose and in RbCl. Furthermore, purified virus labeled with P 32 was efficiently adsorbed to goose red blood cells under optimal conditions for hemagglutination. Chemical analyses showed that Sindbis virus is an RNA virus with a large amount of phospholipid and cholesterol. The virus differs markedly from the host cell both in RNA base ratios and in phosphatide ratios.
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