Abstract
Cyclooxygenases (EC 1.14.99.1) is enzyme family that produces inflammatory prostaglandins, also known asprostaglandin H synthase (PGHS) or prostaglandin-endoperoxide synthase, is expressed in several tissues. COX activity has beendetected in both brain and kidney of mice and bovine kidney (that showed the highest activity). Optimum biochemical propertiesof enzyme activity including incubation time, enzyme and substrate concentrations and pH were determined. Kinetic parametersof COX activity were indicated that the Vmax and Km values were of 66.66 μmole/min and 125.73 μM-1, respectively. COX hasbeen purified from bovine kidney by detergent solubilization, (NH4)2SO4 precipitation, dialysis and ion exchange chromatographyon DEAE-sepharose. Three isoforms were obtained (coxI, coxII and coxIII) that showed specific activities of 592.27, 1323.3 and1825 U/mg protein and 0.72, 1.6 and 2.2 folds purification over the crude homogenate, respectively. The purified enzymeexhibited a single protein band on Coomassie Brilliant Blue stained SDS-PAGE gel corresponding a molecular weight 70 KDa.
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