Abstract
Follicle-stimulating ho:rmone-releasing factor (FSH-RF) activity in lyophilized acetic acid extracts of hypothalamic tissue of porcine origin was concentrated by re-extraction with glacial acetic acid and then subjected to purification by several successive methods. The fractionation procedure consisted of gel filtration on Sephadex G-25, concentration and desalting by phenol extraction, chromsitography and rechromatography on carboxymethylcellulose and column electrophoresis. FSH-RF activity was measured by depletion of pituitary FSHcontent in castrated male rats pretreated with testosterone as well as in normal male rats. In these experiments highly purified FSH-RF was active in vivo at doses of the order of 10 nanograms. These fractions also released FSH from isolated rat pituitaries incubated in vitro. Purified FSH-RF was free of luteinizing hormone-releasing factor (LRF) and other known releasing factors. The response to FSH-RF was not blocked by actinomycin D nor by antihistamines. (Endocrinology81: 882, 1967)
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