Purification and Identification of Total Flavonoids Extracted from Moringa oleifera Leaves in Iraq

Abstract

Moringa oleifera is an important medicinal plant, which contains a lot of bioactive compounds. The dried plant leaves were extracted in 70% methanol by maceration using shaker incubator at 40◦C. Chemical detection of bioactive compounds in crud plant extracts was performed. The total flavonoid was isolated from the extract using reflux, The use of thin layer chromatography technique that assisted in the detection of Qurciten, Rutin and Luteoline of flavonoids isolated from the extract, the existence of qurciten and rutin confirmed by the use of high performance liquid chromatography technique. The calculation of total flavonoid of M. oleifera leaf extracts by using Spectrophotometric technique was done. The aglycon moiety was extracted by ethyl acetate, and then evaporated to dryness. The dried residue then redissolved in 50% ethanol.