PURIFICATION AND EXTRACTION OF POLYSACCHARIDE SPECIFIC OF STREPTOCOCCUS PYOGENES OF GROUP A FIXING ON LATEX PARTICLES

Abstract

The Streptococci are widely distributed in nature and frequently form part of the normal human flora which are members. Approximately 5-15% of humans carry Streptococcus pyogenes. Cytoplasmic membrane has antigens similar to those of human cardiac and heart valve fibroblasts. Patients may also develop immune- mediated such as acute rheumatic fever skin infections and endocarditis. S. pyogenes evolved anenzyme that specifically target IgG use as biochemical reactions, and serologic specificity. Serologic grouping is based on antigenic differences in cell wall carbohydrates (groupA). A technique for detection of antibodies against S. pyogenesgroup antigen / type antigens is being devised as one of the procedures possibly useable in prevention of infections and manufacturers in development of diagnostic and identification tools. Method used for detection of antibodies ( latex agglutination ) are being standardised with antibody levels has been introduced. The detection of antibodies induced by conjugated is under development for antibiotic resistance of Streptococcus pyogenes prepared and provided freeze-dried reference type cultures of Streptococci. The stability of the obtained component subjected at 4 °C and 37 °C over one period to reveal several groups of serum representing healthy population ( N = 20 ) and patients ( N = 40 ) as a significant correlation for the diagnostic of acute rheumatic arthritis. We have shownthat the total amount of specific antibodies does not necessary correlate with the level of neutralizing antibodies, and antibody quality might be clinically more important than antibodyquantity