PURIFICATION AND CONCENTRATION OF GDVII STRAIN OF MOUSE ENCEPHALOMYELITIS VIRUS II. REPORT. PURIFICATION AND CONCENTRATION OF GDVII VIRUS BY MEANS OF RED CELL STROMA

Abstract

Since a procedure of purification and concentration of virus by means of adsorption to and elution from erythrocytes is a simple and effective method, some experiments of purification of pneumotropic and neurotropic viruses capable of hamegglutination have been made by this method. The studies on purification and concentration of the GD VII strain of mouse encephalomyelitis virus were described by Lahelle and Ward, (1) and Nakagawa and Nakamura (2) (3) in recent years. However, thatt the concentration of the virus by this procedure did not produce satisfactory yields of virus because of hemolysis appearing in the process of concentration of the virus by elution from erythrocytes, and that the combination of the methods by means of methanol and acetone precipitation at low temperature with the method of hemagglutination was attempted for obtaining more satisfactory results, were reported in previous papers (2) (3). The purpose of the present paper is to report the research on further more effective method by means of adsorption to and elution from the red cells stroma (4) which are capable of adsorption of viruses.