Abstract
Rate zonal density gradient centrifugation of preparations of Shope papilloma virus revealed three components (slow, middle, and fast) which were morphologically and serologically related. The slow sedimenting component was noninfectious, contained little or no DNA, and had a buoyant density of 1.29 in CsCl. The middle and fast sedimenting components were infectious and had the same buoyant density of 1.34 in CsCl. The middle sedimenting component, yielding a single band upon equilibrium sedimentation in CsCl, was found upon analysis to contain protein and 12% DNA, but no essential lipid and no detectable polyamines. Protein end-group analyses by treatment with hydrazine or with carboxypeptidase A showed the carboxyl terminal amino acid residue to be threonine. The amino-terminal group was unreactive toward fluorodinitrobenzene. The viral protein was analyzed for total amino acid content. Infectious DNA was extracted from the virus by treatment with 80% phenol, and shown to be free of contaminating virus by equilibrium density gradient centrifugation in CsCl.
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