Abstract

rogram. Sequence analysis has demonstrated several mutation ites (18–30) in selected three samples. The second generation as obtained by applying error-prone PCR to the first generation mutated three samples). The changes in catalytic activity of abfa hich is the mutated products of two generations are now under nvestigation. DNA shuffling procedure will be constructed by two ene libraries that consist of twoerror-pronePCR. Thebestmutants ill be generated with high catalytic activity of abfa. oi:10.1016/j.copbio.2011.05.262

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