Abstract
Abstract Solvent tolerant extracellular lipase from Bacillus sphaericus MTCC 7542 was purified by DEAE–Sepharose anion exchange chromatography. This purification resulted in 377 U/mg specific activity and 17.33-fold with 5.7% recovery obtained. The molecular weight of the purified lipase was determined to be 69 kDa using SDS polyacrylamide gel electrophoresis and conformed by zymogram. The purified lipase showed optimal activity and stability at pH 8.0 and 40 °C. The purified lipase was stable in the presence of hydrophobic solvents like n -butanol, benzene, hexane and toluene. Lipase activity was promoted in the presence of Mg 2+ , Ca 2+ , Cu 2+ , K + and Tween 80.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.