Purification and Characterization of New Alkaline L-methioninase from Aspergillus ustus AUMC 1051 Grown under Solid-State Fermentation Conditions.

Abstract

bran) under solid state fermentation (SSF) of wheat bran. The enzyme was purified 15.83-fold with 62.63% yield after three steps of purification involved ammonium sulfate precipitation, Sephadex G-100 gel filtration and DEAE-cellulose ion exchange chromatography. The purified enzyme had a molecular mass of 46 kDa under denaturating conditions and an isoelectric point of 6. Maximal activity was recorded at pH 8.5 and 35